Resequencing of Anaplasma ovis - SRA

SRX3427715: Resequencing of Anaplasma ovis
1 ILLUMINA (Illumina HiSeq 2000) run: 7.3M spots, 3.1G bases, 1.9Gb downloads

Design: Purified DNA samples were sheared into smaller fragments with the desired sizes by Covaris S/E210 or Bioruptor first. Then the overhangs resulting from fragmentation were converted into blunt ends by using T4 DNA polymerase, Klenow Fragment, and T4 Polynucleotide Kinase. After adding an ¡®A¡¯ base to the 3' end of the blunt phosphorylated DNA fragments, adapters were then ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR. The index tags were introduced into the adapter at the PCR stage and a library quality test was performed. At last, the qualified library would be used for sequencing, and the generated data were used for the downstream bioinformatics analysis.

Submitted by: IREC-SaBiO

Study: Draft genome sequence of Anaplasma phagocytophilum, A. marginale and A. ovis isolates with different host and geographical origin

PRJNA420172 • SRP125792 • All experiments • All runs

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Here we report the draft genome sequence of isolates of Anaplasma phagocytophilum, A. marginale and A. ovis. The genome of three A. phagocytophilum (human, cattle and sheep), one A. marginale (cattle) and one A. ovis (sheep) isolates with different host and geographical origin were sequenced and characterized.

Sample:

SAMN08107864 • SRS2719167 • All experiments • All runs

Organism: Anaplasma ovis

Library:

Name: Ao

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 7.3M spots, 3.1G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR6328079	7,341,536	3.1G	1.9Gb	2017-11-29

ID:: 4778303

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