Mitogenomic sequences effectively recover relationships within Nympha... - SRA

SRX534461: Mitogenomic sequences effectively recover relationships within Nymphalidae butterflies
1 LS454 (454 GS Junior) run: 13,049 spots, 8M bases, 17Mb downloads

Design: Thirty entire mitogenomes were amplified by two fragments, one for ~6.5kb in length and the other of ~9.5kb in length. PCR products were checked using 1-2% agarose gel, and each product was purified using commercial purification kit. The two long PCR products were mixed in equimolar concentrations, and then sheared into 200-1000 bp. Each sample was ligated with Roche adaptors and barcode-tag. Total of 30 tagged-samples were mixed together and sequenced using the Roche 454 GS Junior System. Tags and species infomration are listed in below, and more information can be obtained in when our submitted manuscript is accepted. Barcode 5'-ACACAGTGAGT SRS603178 Athyma kasa Barcode 5'-CGACGAGTACT SRS603155 Euploea core Barcode 5'-CAGTACGTACT SRS603157 Parantica sita Barcode 5'-AGTCGTACACT SRS603159 Dichorragia nesimachus Barcode 5'-ACTATACGAGT SRS603160 Yoma sabina Barcode 5'-AGTACTACTAT SRS603161 Junonia almana Barcode 5'-CGATACTACGT SRS603162 Argynnis childreni Barcode 5'-AGTAGACGTCT SRS603163 Polyura arja Barcode 5'-AGTACGAGAGT SRS603164 Melanitis phedima Barcode 5'-ACGACACGTAT SRS603165 Ypthima akragas Barcode 5'-ATAGTATACGT SRS603166 Neope pulaha Barcode 5'-CGACGACGCGT SRS603167 Bhagadatta austenia Barcode 5'-GTACAGTACGT SRS603168 Parthenos sylvia Barcode 5'-ACGTAGATCGT SRS603169 Neptis soma Barcode 5'-GTGTACGACGT SRS603170 Neptis philyra Barcode 5'-ACTACGTCTCT SRS603171 Pantoporia hordonia Barcode 5'-ACACGTAGTAT SRS603172 Euthalia irrubesens Barcode 5'-CGTACGTCGAT SRS603173 Tanaecia julii Barcode 5'-ACGTACACACT SRS603174 Lexias dirtea Barcode 5'-ACGTACTGTGT SRS603175 Dophla evelina Barcode 5'-ACTCGCGTCGT SRS603176 Abrota ganga Barcode 5'-GTCGTACGTAT SRS603177 Athyma opalina Barcode 5'-AGTGTAGTAGT SRS603179 Athyma asura Barcode 5'-ACACGACGACT SRS603180 Athyma cama Barcode 5'-ACACTACTCGT SRS603181 Athyma perius Barcode 5'-CTACTCGTAGT SRS603182 Sumalia daraxa Barcode 5'-ACGCGTCTAGT SRS603183 Pandita sinope Barcode 5'-AGACTCGACGT SRS603184 Parasarpa dudu Barcode 5'-ACACTCATACT SRS603939 Athyma selenophora specimen 1 Barcode 5'-ACGAGTAGACT SRS603940 Athyma selenophora specimen 2

Submitted by: NATIONAL TAIWAN UNIVERSITY

Study: Mitogenomic sequences effectively recover relationships within Nymphalidae butterflies

PRJNA246341 • SRP041730 • All experiments • All runs

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Mitogenomic phylogenies have revealed well supported relationships for many groups of eukaryotes, including Lepidoptera. However, these data is restricted to 7 of 43 known superfamilies, and it remains challenging to recover large numbers of mitogenomes at once. Therefore, we present a procedure to obtain a large number of mitogenomes via the Roche 454 GS junior system.

Sample: Model organism or animal sample from Parasarpa dudu

SAMN02756807 • SRS603184 • All experiments • All runs

Organism: Parasarpa dudu

Library:

Instrument: 454 GS Junior

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 13,049 spots, 8M bases, 17Mb

Run	# of Spots	# of Bases	Size	Published
SRR1273419	13,049	8M	17Mb	2014-06-17

ID:: 760288

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