Nemapogon cloacella transcriptome - SRA

SRX1603924: Nemapogon cloacella transcriptome
1 ILLUMINA (Illumina HiSeq 1000) run: 22.8M spots, 4.6G bases, 2.7Gb downloads

Design: Nucleic acids were extracted using Promega SV total RNA isolation mini-kits either with or without DNase digestion. Following DNase digestion, the RNA-only preps were subjected to poly-A selection and indexed library construction. The remaining extracts of total nucleic acids were used to produce cDNAs with low-input Clontech kits for either poly-dT priming (SMARTer Ultra Low input RNA kit Đv3, #634849) or universal priming (#634940). Following shearing to 200-bp size with a Covaris instrument, cDNA fragments were used for indexed library construction (Clontech kit #634947 for low input). Libraries were left unnormalized so as to favor highly expressed genes likely to be present in most species and all life stages.

Submitted by: University of Maryland

Study: Leptree II: tineoid transcriptomes

PRJNA312833 • SRP070913 • All experiments • All runs

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This study uses RNA-Seq to resolve phylogenetic relationships in the insect order Lepidoptera (moths and butterflies). In particular, it seeks to determine whether this approach can conclusively resolve tineoid relationships.

Sample: Nemapogon cloacella transcriptome

SAMN04517255 • SRS1313701 • All experiments • All runs

Organism: Nemapogon cloacella

Library:

Name: Nclo

Instrument: Illumina HiSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 22.8M spots, 4.6G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR3193089	22,775,215	4.6G	2.7Gb	2018-03-01

ID:: 2293985

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