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SRX8934637: Whole genome sequencing of Consolea nashii: epidermal tissue
1 ILLUMINA (Illumina HiSeq 2000) run: 9.2M spots, 2.8G bases, 1Gb downloads

Design: Genomic DNA was extracted from epidermal tissue using a modified CTAB extraction protocol. Extracted DNA was sent to RAPiD Genomics (Gainesville, FL, USA) for shotgun library preparation and 2 x 150 bp paired-end Illumina HiSeq sequencing.
Submitted by: University of Florida
Study: Pleistocene aridification underlies the evolutionary history of the Caribbean endemic, insular giant, Consolea (Cactaceae, Opuntioideae)
show Abstracthide Abstract
The Caribbean islands are renowned for their small size but high species diversity, and cacti make up a fascinating component of seasonally dry tropical forest (SDTF) there. Consolea consist of nine species of dioecious, hummingbird pollinated trees endemic to the West Indies, which form a conspicuous element of the SDTF. Several species are threatened by anthropogenic disturbance, disease, sea-level rise and invasive species, and are of conservation concern. However, no comprehensive phylogeny yet exists for the clade. We reconstructed the phylogeny of Consolea, sampling all species using plastome data to determine relationships, understand the evolution of key morphological characters and test their biogeographic history. We estimated divergence times to determine the role climate change may have played in shaping the current diversity of the clade. Consolea appears to have evolved very recently during the latter part of the Pleistocene on Cuba likely from a South American ancestor, and from there moved into the Bahamas, Hispaniola, Jamaica, Puerto Rico, Florida and the Lesser Antilles. The tree growth form is a synapomorphy of Consolea and likely aided in the establishment and diversification of the clade. Pleistocene aridification associated with glaciation likely played a role in shaping the current diversity of Consolea, and insular gigantism may have been a key innovation leading to the success of these species to invade the often dense SDTF. This in-situ Caribbean radiation provides a window into the generation of species diversity and the complexity of the SDTF community within the Antilles.
SAMN15717103 • SRS7190936 • All experiments • All runs
Organism: Consolea nashii
Name: DBG_1996_0257_01_1
Instrument: Illumina HiSeq 2000
Strategy: WGS
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 9.2M spots, 2.8G bases, 1Gb
Run# of Spots# of BasesSizePublished


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