GSM5899229: aspergillus_WT_2; Aspergillus flavus; RNA-Seq - SRA

SRX14173701: GSM5899229: aspergillus_WT_2; Aspergillus flavus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 26.3M spots, 7.9G bases, 2.4Gb downloads

External Id: GSM5899229_r1

Submitted by: Fujian Agriculture and Forestry University

Study: Regulator of G protein signaling contributes to the development and aflatoxin biosynthesis in Aspergillus flavus through the regulation of Ga activity.

PRJNA806956 • SRP359794 • All experiments • All runs

show Abstracthide Abstract

purpose: to further gain insight into the effect of RGS proteins on aflatoxin biosynthesis in A. flavus NRRL3357. methods: two mutants ?rgsA and ?rgsE separately compared to WT were chose to conduct the analysis of transcriptomics. results: 1750 DEGs are found, including 720 upregulated genes and 1030 downregulated genes in (?rgsA vs WT); aflB, aflD, aflF, aflG, aflN, aflP, aflS from ?rgsE were transcriptionally reduced relative to ?rgsA in their expressions. Overall design: mRNA profiles of wild type (WT), ?rgsA and ?rgsE.

Sample: aspergillus_WT_2

SAMN25943193 • SRS11997657 • All experiments • All runs

Organism: Aspergillus flavus

Library:

Name: GSM5899229

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The harvested-mycelia (WT, ΔrgsA and ΔrgsE) were frozen in liquid nitrogen, and were lyophilized for 1day. The RNA of WT, ΔrgsA and ΔrgsE strains was extracted by TRIzol reagent according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols.

Runs: 1 run, 26.3M spots, 7.9G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR18019559	26,280,324	7.9G	2.4Gb	2022-02-21

ID:: 19979791

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity