GSM722423: CEBPA_U937bio_r1_100709_5 - SRA

SRX097095: GSM722423: CEBPA_U937bio_r1_100709_5
1 ILLUMINA (Illumina Genome Analyzer II) run: 21.2M spots, 764.3M bases, 472.9Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)

PRJNA142955 • SRP008215 • All experiments • All runs

show Abstracthide Abstract

Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human ChIP-seq portion of this dataset on leukemia cell lines. Overall design: Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008), CEBPA (SC-9314) or H3K4me1 (Abcam ab8895).

Sample: CEBPA_U937bio_r1_100709_5

SAMN00717427 • SRS261009 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM722423: CEBPA_U937bio_r1_100709_5

Instrument: Illumina Genome Analyzer II

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Spot descriptor:

1 forward

Experiment attributes:

GEO Accession: GSM722423

Links:

External link: GEO Web Link

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 21.2M spots, 764.3M bases, 472.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR343314	21,230,457	764.3M	472.9Mb	2011-10-11

ID:: 111591

SRA

Sequence Read Archive

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