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SRX1055081: Illumina shotgun metagenomic sequencing: Sample LIG_BR2_2012
1 ILLUMINA (Illumina HiSeq 1000) run: 15.5M spots, 3.3G bases, 1.9Gb downloads

Design: "We used Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA was sheared using a Covaris and libraries were constructed with the Nugen Ovation Ultralow Library protocol and were amplified for 11 cycles. The amplified product was visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay. Libraries were pooled at equimolar concentrations based on these results and size selected using a Sage PippinPrep 2% cassette. The final library pool has an average insert size of 170bp. it was quantified using a Kapa Biosystems qPCR library quantification kit, then sequenced on the Illumina HiSeq1000 in a 2x101 paired-end sequencing run using dedicated read indexing. The samples were demultiplexed with CASAVA 1.8.2."
Submitted by: Marine Biological Laboratory
Study: Serpentinizing microbial ecosystems Metagenome
show Abstracthide Abstract
Ultramafic rocks in the Earth’s mantle represent a tremendous reservoir of carbon and reducing power. Upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and provides opportunities for subsurface microbial communities to interact with deep carbon pools.
Sample: Voltri Massif travertine
SAMN03761830 • SRS957806 • All experiments • All runs
Library:
Name: LIG_BR2
Instrument: Illumina HiSeq 1000
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
forward109  reverse

Experiment attributes:
experiment_number: 1
Runs: 1 run, 15.5M spots, 3.3G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR205840515,452,0303.3G1.9Gb2016-05-03

ID:
1531854

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