WGS project for strain CGII-47 Alcaligenaceae bacterium - SRA

SRX11311979: WGS project for strain CGII-47 Alcaligenaceae bacterium
1 ILLUMINA (Illumina MiSeq) run: 842,790 spots, 421.4M bases, 227.9Mb downloads

Design: For the whole genome project of strain CGII-47 the genomic DNA was extracted with the DNeasy PowerLyzer Microbial Kit (Qiagen) including an RNase A treatment at 37 C for 20 min. Illumina sequencing was performed by the Genomics Facility RTSF, Michigan State University (USA) with the following main steps: library preparation using the SMARTer ThruPLEX DNA-Seq kit (Takara); quality control using a combination of Qubit dsDNA HS assay (Thermo Fisher Scientific), 4200 TapeStation High Sensitivity DNA 1000 assay (Agilent) and the Illumina Library Quantification qPCR kit (Kapa Biosystems); sequencing which was performed in a 2x250 bp paired-end format using a MiSeq Standard v2 flow cell and a MiSeq 500 cycle v2 reagent cartridge (Illumina).

Submitted by: ELTE Eotvos Lorand University

Study: Taxonomic revisions and descriptions of new bacterial species based on genomic comparisons in the high-G+C-content subgroup of the family Alcaligenaceae

PRJNA626537 • SRP272322 • All experiments • All runs

show Abstracthide Abstract

De novo assembled genomes of unknown and already described species of the Alcaligenaceae family

Sample:

SAMN19931335 • SRS9341028 • All experiments • All runs

Organism: Alcaligenaceae bacterium CGII-47

Library:

Name: Alcaligenaceae bacterium CGII-47

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM