Microbe sample from Clostridium ljungdahlii PETC - SRA

SRX992185: Microbe sample from Clostridium ljungdahlii PETC
1 ILLUMINA (Illumina MiSeq) run: 9.7M spots, 3.8G bases, 1.6Gb downloads

Design: An UltraClean Microbial DNA Isolation Kit (MoBio, Carlsbad, CA) was used for DNA extraction from 10 mL serum bottle cultures grown with syngas as described above until late-exponential growth stage. Due to low initial DNA concentrations, the MoBio protocol was modified to include a 10-minute, 70 degree Celsius heat shock step followed by a 10 minute vortex step. A NanoDrop 2000c UV-Vis spectrophotometer (ThermoSCIENTIFIC, Wilmington DE) was used to determine quality and concentration (20 -50 ng•µL-1) of isolated DNA. Samples were stored at -20°C until use.

Submitted by: (Cornell)

Study: Syngas fermenting Clostridium genomes

PRJNA280340 • SRP057084 • All experiments • All runs

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The genomes of C. ljungdahlii strains PETC and ERI-2, C. autoethanogenum JA1-1, and C. coskatii PS02 were sequenced in order to analyze phenotypic differences that were observed during syngas-to-ethanol fermentation.

Sample: Microbe sample from Clostridium ljungdahlii PETC

SAMN03459581 • SRS906955 • All experiments • All runs

Organism: Clostridium ljungdahlii DSM 13528

Library:

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward251 reverse

Runs: 1 run, 9.7M spots, 3.8G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR1970388	9,652,734	3.8G	1.6Gb	2015-08-28

ID:: 1439961

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