Shotgun sequencing of water sample from a Patagonian fjord (marine me... - SRA

SRX10857652: Shotgun sequencing of water sample from a Patagonian fjord (marine metagenome): 20 meters deep
1 ILLUMINA (Illumina HiSeq 3000) run: 29.1M spots, 5.9G bases, 1.9Gb downloads

Design: We filtered 25L of 20-meter deep marine sample from Comau fjord through a 0.22 micron Sterivex to capture bacterial cells. Then, we extracted DNA from the 0.22 um filter through phenol-chloroform DNA extraction methodology, using Xanthogenate buffer for lysis step. Sequencing library were prepared using Illumina TruSeq DNA PCR-Free kit, following its sample preparation guide.

Submitted by: Universidad Andres Bello

Study: Microbial Ecology of the Comau Fjord, Northern Patagonia (42 S)

PRJNA729490 • SRP319507 • All experiments • All runs

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This project seeks to understand how microbial communities respond to anthropogenic disturbances and how this could provide insights into microbial ecology and evolution. We also seek to understand and quantify the impact and reach of human activities and its consequences on environmental and human health.

Sample:

SAMN19118246 • SRS8953146 • All experiments • All runs

Organism: marine metagenome

Library:

Name: DEC2017_L3S3D20

Instrument: Illumina HiSeq 3000

Strategy: WGS

Source: METAGENOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 29.1M spots, 5.9G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR14511855	29,101,180	5.9G	1.9Gb	2021-05-15

ID:: 14440760

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