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SRX10857580: Shotgun sequencing of water sample from a Patagonian fjord (marine metagenome): 5 meters deep
1 ILLUMINA (Illumina HiSeq 3000) run: 20.8M spots, 4.2G bases, 1.5Gb downloads

Design: We filtered 20-25L of 5-meter deep marine sample from Comau fjord through a 0.22 micron Sterivex to capture bacterial cells. Then, we extracted DNA from the 0.22 um filter through phenol-chloroform DNA extraction methodology, using Xanthogenate buffer for lysis step. Sequencing library were prepared using Illumina TruSeq DNA PCR-Free kit, following its sample preparation guide.
Submitted by: Universidad Andres Bello
Study: Microbial Ecology of the Comau Fjord, Northern Patagonia (42 S)
show Abstracthide Abstract
This project seeks to understand how microbial communities respond to anthropogenic disturbances and how this could provide insights into microbial ecology and evolution. We also seek to understand and quantify the impact and reach of human activities and its consequences on environmental and human health.
Sample:
SAMN19118269 • SRS8953074 • All experiments • All runs
Library:
Name: EC-BacI
Instrument: Illumina HiSeq 3000
Strategy: WGS
Source: METAGENOMIC
Selection: size fractionation
Layout: PAIRED
Runs: 1 run, 20.8M spots, 4.2G bases, 1.5Gb
Run# of Spots# of BasesSizePublished
SRR1451192720,763,9604.2G1.5Gb2021-05-14

ID:
14440688

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