Hydrothermal vent biofilm microbial shotgun metagenome - SRA

SRX748869: Hydrothermal vent biofilm microbial shotgun metagenome
1 ILLUMINA (Illumina HiSeq 1000) run: 5.5M spots, 1.2G bases, 727.1Mb downloads

Design: "We used Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA was sheared using a Covaris and libraries were constructed with the Nugen Ovation Ultralow Library protocol and were amplified for 15 cycles. The amplified product was visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay. Libraries were pooled at equimolar concentrations based on these results and size selected using a Sage PippinPrep 2% cassette. The final library pool has an average insert size of 170bp. it was quantified using a Kapa Biosystems qPCR library quantification kit, then sequenced on the Illumina HiSeq1000 in a 2x101 paired-end sequencing run using dedicated read indexing. The samples were demultiplexed with CASAVA 1.8.2."

Submitted by: Marine Biological Laboratory

Study: Serpentinizing microbial ecosystems Metagenome

PRJNA265986 • SRP049438 • All experiments • All runs

show Abstracthide Abstract

Ultramafic rocks in the Earth’s mantle represent a tremendous reservoir of carbon and reducing power. Upon tectonic uplift and exposure to fluid flow, serpentinization of these materials generates copious energy, sustains abiogenic synthesis of organic molecules, and provides opportunities for subsurface microbial communities to interact with deep carbon pools.

Sample: Hydrothermal vent biofilm

SAMN03154341 • SRS734862 • All experiments • All runs

Organism: aquatic metagenome

Library:

Instrument: Illumina HiSeq 1000

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward109 reverse

Runs: 1 run, 5.5M spots, 1.2G bases, 727.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1636516	5,507,502	1.2G	727.1Mb	2015-10-31

ID:: 1082819

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