WGS project for strain CAII-53 Alcaligenaceae bacterium - SRA

SRX8753322: WGS project for strain CAII-53 Alcaligenaceae bacterium
1 ILLUMINA (Illumina MiSeq) run: 1.6M spots, 785M bases, 407.4Mb downloads

Design: For the whole genome project of strain CAII-53, the genomic DNA was extracted with the DNeasy PowerLyzer Microbial Kit (Qiagen) including an RNase A treatment at 37 C for 20 min. Illumina sequencing was performed by the Genomics Facility RTSF, Michigan State University (USA) with the following main steps: library preparation using the SMARTer ThruPLEX DNA-Seq kit (Takara); quality control using a combination of Qubit dsDNA HS assay (Thermo Fisher Scientific), 4200 TapeStation High Sensitivity DNA 1000 assay (Agilent) and the Illumina Library Quantification qPCR kit (Kapa Biosystems); sequencing which was performed in a 2x250 bp paired-end format using a MiSeq Standard v2 flow cell and a MiSeq 500 cycle v2 reagent cartridge (Illumina).

Submitted by: ELTE Eotvos Lorand University

Study: Taxonomic revisions and descriptions of new bacterial species based on genomic comparisons in the high-G+C-content subgroup of the family Alcaligenaceae

PRJNA626537 • SRP272322 • All experiments • All runs

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De novo assembled genomes of unknown and already described species of the Alcaligenaceae family

Sample:

SAMN15438393 • SRS7025339 • All experiments • All runs

Organism: Alcaligenaceae bacterium

Library:

Name: Alcaligenaceae bacterium CAII-53

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM