WGS project for strain Pusillimonas soli DSM 25264 bacterium - SRA

SRX8753321: WGS project for strain Pusillimonas soli DSM 25264 bacterium
1 ILLUMINA (Illumina MiSeq) run: 1.7M spots, 826.5M bases, 433.6Mb downloads

Design: For the whole genome project of strain DSM 25264, the genomic DNA was extracted with the DNeasy PowerLyzer Microbial Kit (Qiagen) including an RNase A treatment at 37 C for 20 min. Illumina sequencing was performed by the Genomics Facility RTSF, Michigan State University (USA) with the following main steps: library preparation using the SMARTer ThruPLEX DNA-Seq kit (Takara); quality control using a combination of Qubit dsDNA HS assay (Thermo Fisher Scientific), 4200 TapeStation High Sensitivity DNA 1000 assay (Agilent) and the Illumina Library Quantification qPCR kit (Kapa Biosystems); sequencing which was performed in a 2x250 bp paired-end format using a MiSeq Standard v2 flow cell and a MiSeq 500 cycle v2 reagent cartridge (Illumina).

Submitted by: ELTE Eotvos Lorand University

Study: Taxonomic revisions and descriptions of new bacterial species based on genomic comparisons in the high-G+C-content subgroup of the family Alcaligenaceae

PRJNA626537 • SRP272322 • All experiments • All runs

show Abstracthide Abstract

De novo assembled genomes of unknown and already described species of the Alcaligenaceae family

Sample: type strain

SAMN15438384 • SRS7025338 • All experiments • All runs

Organism: Allopusillimonas soli

Library:

Name: Pusillimonas soli DSM 25264

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM