U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX6070865: RNA-seq of equine intestinal microbiome
1 ILLUMINA (Illumina MiSeq) run: 77,532 spots, 38.9M bases, 23.2Mb downloads

Design: field-study | case-control | rectal swab samples | V4 Region of the bacterial 16S rRNA gene | Illumina MiSeq
Submitted by: University of Bern
Study: Equine Intestinal Microbiome in Hypersensitivity Disorders
show Abstracthide Abstract
Background: Recent evidence suggests that an altered intestinal microbiome, namely a reduction of bacterial diversity or a shift in microbial composition, is associated with the development of hypersensitivity diseases in humans but this is unknown for horses.Hypothesis: We hypothesized that horses affected by either Culicoides hypersensitivity (CH) or severe equine asthma (SEA) or both show a decreased diversity of their intestinal microbiome in comparison to healthy peers.Methods: Rectal swab samples of a total of 140 horses were collected. Horses were divided into three groups. “CH” comprised 30 horses affected by Culicoides hypersensitivity, group “SEA” consisted of 30 horses suffering from severe equine asthma and group “CH-SEA” comprised 10 horses affected by both aforementioned diseases. For each allergic horse a healthy peer from the same stable was similarly sampled as a matched control. Microbiota in the swabs were determined by assessing the V4 region of the bacterial 16S rRNA gene by means of Illumina MiSeq. Structures of bacterial communities were investigated by means of alpha and beta diversity indices.
Sample:
SAMN12057600 • SRS4973085 • All experiments • All runs
Library:
Name: 134Ne
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: METAGENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 77,532 spots, 38.9M bases, 23.2Mb
Run# of Spots# of BasesSizePublished
SRR930208577,53238.9M23.2Mb2019-07-25

ID:
8115605

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...