Spiroplasma poulsonii MSRO-BK genome - SRA

SRX5741322: Spiroplasma poulsonii MSRO-BK genome
1 PACBIO_SMRT (PacBio RS II) run: 163,482 spots, 1.1G bases, 3.8Gb downloads

Design: S. poulsonii strain MSRO-BK DNA was extracted from fly hemolymph as previously described. Processing of the samples was performed in the University of Lausanne Genomic Technologies Facility. The DNA was sheared in a Covaris g-TUBE (Covaris, Woburn, MA, USA) to obtain 20 kb fragments. After shearing the DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies, Ames, IA, USA). 2.1 g of the sheared DNA was used to prepare a SMRTbell library with the PacBio SMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's recommendations. The resulting library was size selected on a BluePippin system (Sage Science, Inc. Beverly, MA, USA) for molecules larger than 6 kb. The recovered library was sequenced on one SMRT cell with P6/C4 chemistry and MagBeads on a PacBio RSII system (Pacific Biosciences, Menlo Park, CA, USA) at 240 min movie length.

Submitted by: University of Lausanne

Study: Spiroplasma poulsonii Genome sequencing and assembly

PRJNA256019 • SRP092604 • All experiments • All runs

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Spiroplasma poulsonii MSRO genome sequencing

Sample:

SAMN11349289 • SRS4676830 • All experiments • All runs

Organism: Spiroplasma poulsonii

Library:

Name: MSROBK_SSBE00000000

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 163,482 spots, 1.1G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR8961770	163,482	1.1G	3.8Gb	2020-05-05

ID:: 7729409

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