WGS of Micromonospora sp. MW-13: TruSeq PCR-free library - SRA

SRX4783691: WGS of Micromonospora sp. MW-13: TruSeq PCR-free library
1 ILLUMINA (Illumina HiSeq 1500) run: 2.6M spots, 1.3G bases, 632.5Mb downloads

Design: For the isolation of gDNA, the culture was grown in 15 ml TSB (Tryptic Soy Broth) overnight at 30C on a rotary shaker (180 rpm). For gDNA isolation, the QIAGEN Genomic DNA Purification kit in combination with 100/G Genomic-tips (QIAGEN) were used according to the manufacturers protocol, except that for the bacterial lysis the handled volumes were doubled and incubation time at 50C was prolonged until a clear lysate was obtained.A paired-end library constructed (TruSeq PCR-free Library Kit) and subjected to sequencing using a Illumina HiSeq 1500 platform.

Submitted by: Bielefeld University

Study: Micromonospora sp. MW-13 strain:MW13 Genome sequencing and assembly

PRJNA434501 • SRP163191 • All experiments • All runs

show Abstracthide Abstract

Bacteria of the genus Micromonospora are known to be a rich source for bioactive compounds. This study aims therefore at the genome-driven isolation and strucure elucidation of novel secondary metbabolites and their subsequent biological evaluation in diverse assays.

Sample: Micromonospora sp. MW13

SAMN08562404 • SRS3870297 • All experiments • All runs

Organism: Micromonospora sp. MW-13

Library:

Name: Sample_60

Instrument: Illumina HiSeq 1500

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.6M spots, 1.3G bases, 632.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR7949780	2,602,577	1.3G	632.5Mb	2018-10-03

ID:: 6468998

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