SRX3989431: MG_DIP2
1 ILLUMINA (NextSeq 500) run: 7.6M spots, 2.3G bases, 898.4Mb downloads
Design: 100 ng of DNA per samples was sheared to approximately 250300 bp (Covaris M2). Sheared DNA was end-repaired and A-tailed with a single A dNTP, the NEB adapter (NEB #E7645L) was then ligated to the end-repaired A-tailed DNA fragment. Size selection of the library was performed using Agencourt AMPure XP beads (Beckman Coulter) for size selection of 250 bp fragment inserts. Dual indexes were used in the library enrichment step to add Illumina TruSeq HT indexes (NEB #7600) to the DNA molecules using 6 cycles of PCR. Libraries were quality checked using the Bioanalyzer DNA 1000 Chip (Agilent Technologies), quantified using the Qubit BR DNA system (Invitrogen), and subsequently pooled in equimolar ratios to a final concentration of 10 nM. The pool was re-quantified using qPCR (KAPA Biosystems library quantification) with the ABI HT7900 (Applied Biosystems) and sequenced on the Illumina NextSeq 500 at 1.8 pM with 1 % PhiX.
Submitted by: KAUST
Study:
Red Sea coral metagenomesshow Abstracthide AbstractHere we generated metagenomes from a suite of Red Sea corals to assess microbial diversity.
Library:
Name: MG_DIP2
Instrument: NextSeq 500
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 7.6M spots, 2.3G bases, 898.4Mb