Genome sequencing of Rathayibacter rathayi: strain Z015 - SRA

SRX3631426: Genome sequencing of Rathayibacter rathayi: strain Z015
1 ILLUMINA (Illumina HiSeq 3000) run: 2.5M spots, 740.1M bases, 299.9Mb downloads

Design: DNA was extracted using the DNeasy Blood and Tissue kits (QIAGEN, Valencia, California), following instructions for Gram-positive bacteria. A NanoDrop ND-1000 UV-Vis Spectrophotometer and Qubit 2.0 fluorometer (ThermoFisher Scientific, Waltham, MA, USA) were used to measure the quality and quantity, respectively, of the DNA. Libraries were prepared, using the Illumina Nextera XT library preparation protocol and sequenced on the Illumina HiSeq 3000 by the Center for Genome Research and Biocomputing (CGRB at Oregon State University; Illumina Inc., San Diego, CA, USA).

Submitted by: Oregon State University

Study: Rathayibacter toxicus, Rathayibacter iranicus, Rathayibacter tritici, Rathayibacter rathayi, Clavibacter michiganensis, Pseudoclavibacter Genome sequencing and assembly

PRJNA432233 • SRP131804 • All experiments • All runs

show Abstracthide Abstract

A population study of Rathayibacter toxicus from Australia, as well as a paired population of a related novel Rathayibacter species primarily found in Oregon. Several other Rathayibacter species were examined as well. The work reveals the ecological role of CRISPR in Rathayibacter toxicus and uses the novel Rathayibacter species as a comparison where a CRISPR-phage interaction is not involved in the evolution of the bacteria.

Sample:

SAMN08438727 • SRS2898157 • All experiments • All runs

Organism: Rathayibacter rathayi

Library:

Name: Z015

Instrument: Illumina HiSeq 3000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.5M spots, 740.1M bases, 299.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR6654073	2,450,677	740.1M	299.9Mb	2018-01-30

ID:: 5026893

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