9683 - SRA - NCBI

SRX3551127: 9683
1 ILLUMINA (Illumina HiSeq 2500) run: 64.2M spots, 12.4G bases, 5.9Gb downloads

Design: Stool samples were homogenized in 1 mL of RNAlater. The NucliSens Magnetic Extraction kit and the QIAamp Stool DNA Miniprep Kit were used to extract nucleic acids from samples total nucleic acids and DNA respectively. Total nucleic acids were initially treated with the TURBO DNA-free kit and purified using the RNeasy MinElute Cleanup kit. For removal of ribosomal RNA from plant bacteria and mammalian host prior to RNA library preparation the total purified RNA was subjected to the ScriptSeq Complete Gold Kit: Epidemiology. The MiniElute PCR Purification Kit and the Agencourt AMPure XP System: PCR Purification kit were used to purify the PCR-amplified products whenever requested by the ScriptSeq kit protocol. DNA libraries were constructed using the DNA directly in Nextera DNA Sample Preparation kit.

Submitted by: LVH

Study: Gorilla fecal virome

PRJNA419744 • SRP128846 • All experiments • All runs

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The aim of this study was to identify and compare enteric viromes of SIV-infected and uninfected gorillas, and to assess their relationship with SIVgor pathogenesis in their natural host.

Sample: 9683

SAMN08143643 • SRS2824517 • All experiments • All runs

Organism: gut metagenome

Library:

Name: CP9683

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: METAGENOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 64.2M spots, 12.4G bases, 5.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR6460877	64,187,328	12.4G	5.9Gb	2018-05-31

ID:: 4931956

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