show Abstracthide AbstractThe gut fungal microbiome of honeybee (Apis mellifera) was investigated to evaluate the composition and spatial organization in different gut portions. Adult foragers were collected in the late summer from experimental apiaries located at DISAFA (University of Turin, Italy). Microbiological analyses were performed on the dissected guts compartments (i.e. crop, midgut, ileum and rectum) of honeybee workers. High-throughput sequencing of ITS2 region were used to investigate the fungal communities associated to the gut compartments of this insect.