Update of Spiroplasma poulsonii MSRO genome - SRA

SRX3446810: Update of Spiroplasma poulsonii MSRO genome
1 PACBIO_SMRT (PacBio RS II) run: 163,482 spots, 530.5M bases, 1.8Gb downloads

Design: Spiroplasma DNA was extracted from fly hemolymph as previously described (Paredes et al. 2015) . Processing of thesamples was performed in the University of Lausanne Genomic Technologies Facility. The DNA wassheared in a Covaris g-TUBE (Covaris, Woburn, MA, USA) to obtain 20 kb fragments. After shearingthe DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies,Ames, IA, USA). 5 µg of the sheared DNA was used to prepare a SMRTbell library with the PacBioSMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to themanufacturer's recommendations. The resulting library was size selected on a BluePippin system(Sage Science, Inc. Beverly, MA, USA) for molecules larger than 20 kb. The recovered library wassequenced on one SMRT cell with P6/C4 chemistry and MagBeads on a PacBio RSII system (PacificBiosciences, Menlo Park, CA, USA) at 240 min movie length.

Submitted by: University of Lausanne

Study: Spiroplasma poulsonii Genome sequencing and assembly

PRJNA256019 • SRP092604 • All experiments • All runs

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Spiroplasma poulsonii MSRO genome sequencing

Sample:

SAMN07737451 • SRS2736763 • All experiments • All runs

Organism: Spiroplasma poulsonii

Library:

Name: MSRO_JTLV02000000

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 163,482 spots, 530.5M bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR6350066	163,482	530.5M	1.8Gb	2018-03-22

ID:: 4804257

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