show Abstracthide AbstractThis project focus on a Denitrifying Candidatus Accumulibacter enrichment culture that we've shown to be capable of producing substantial nitrous oxide when nitrite is present as the electron acceptor for denitrification. Shotgun metagenomic sequencing and genome-resolved metagenome analysis were applied to investigate the genomic nitrogen transformation potential for two Accumulibacter genomes as well as flanking bacterial genomes.