metagenome sequence of total DNA fromAgarose-alginate particle enrich... - SRA

SRX13303380: metagenome sequence of total DNA fromAgarose-alginate particle enrichment 132h timepoint
1 ILLUMINA (Illumina HiSeq 2500) run: 335,112 spots, 167.6M bases, 85.4Mb downloads

Design: Metagenomic sequencing libraries were prepared using genomic DNA extracted from particle samples at 0, 12, 24, 36, 48, 60, 72, 108, 132, 156 and 204 h timepoints. 2x250 paired-end sequencing libraries were prepared with Illumina Nexterra-XT adapters, using a modifcations to the protocol previously validated for low DNA input (Rinke C, Low S, Woodcroft BJ, Raina JB, Skarshewski A, Le XH, et al. Validation of picogram-and femtogram-input DNA libraries for microscale metagenomics. PeerJ. 2016;4:e2486.)

Submitted by: Massachusetts Institute of Technology

Study: Modular assembly of carbohydrate-degrading microbial communities in the ocean

PRJNA478695 • SRP187241 • All experiments • All runs

Sample: metagenome of seawater bacteria enriched on Agarose-alginate beads sampled at 132 hours

SAMN23600617 • SRS11212975 • All experiments • All runs

Organism: marine metagenome

Library:

Name: GCTACGCT-GTAAGGAG-s

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 335,112 spots, 167.6M bases, 85.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR17118787	335,112	167.6M	85.4Mb	2021-12-03

ID:: 18194104

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