Deep hyper saline anoxic basin sediments - SRA

SRX1118955: Deep hyper saline anoxic basin sediments
1 ILLUMINA (Illumina HiSeq 2000) run: 14M spots, 4.2G bases, 3.1Gb downloads

Design: After confirming the absence of DNA, the Ovation Single Cell RNA-seq kit (Nugen, San Carlos, CA) was used to create barcoded cDNA libraries for sequencing. A modified step was followed in which the First Strand Primer Mix, A1 ver 15 (S01882) was replaced with the First Strand Primer Mix, A1 ver 13 (S01858) to avoid biases against prokaryotic transcripts. The libraries were sequenced at DNA Sequencing & Genotyping Center, University of Delaware using Illumina HiSeq platform and paired-end 150 run. The five libraries were pooled in 3 lanes. The forward and reverse reads were filtered using Trimmomatic. The length of the trimmed sequences was set to be at least 50 nt. Surviving trimmed reads were used for assembly with Trinity release r20140717.

Submitted by: Woods Hole Oceanographic Institution

Study: Deep Hypersaline Anoxic Basins Transcriptome or Gene expression

PRJNA291018 • SRP061621 • All experiments • All runs

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This project aims to describe the overall picture of transcribed genes from microorganisms (all 3 domains of life) in sediments underlying the halo clines (transition zones between normal salinity deep mediterranean Sea and brine) of several deep hyper saline anoxic basins in the E. Mediterranean Sea. Average depth is ~3500m or greater. Also some nearby normal salinity core samples were collected around 10m outside the influence of the brines. Analyses are based on top ~3cm of the sediments

Sample: UC

SAMN03760857 • SRS1011626 • All experiments • All runs

Organism: sediment metagenome

Library:

Name: UraniaBasinControlSediments

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: METATRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward152 reverse

Runs: 1 run, 14M spots, 4.2G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR2542010	13,993,879	4.2G	3.1Gb	2016-01-01

ID:: 1632960

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