Complete genome sequence of adherent invasive Escherichia coli UM146 ... - SRA

SRX024861: Complete genome sequence of adherent invasive Escherichia coli UM146 isolated from ileal Crohn’s disease biopsy tissue
1 LS454 (454 GS FLX Titanium) run: 225,673 spots, 118.8M bases, 248.8Mb downloads

Design: Bacteria genomic DNAs were extracted using a QIAamp DNA mini kit (Qiagen Inc.). DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech, Paris, France) and quality evaluated using BioRad Experion labchip system (BioRad). Utilizing good quality 700 ng/ul 1.82 630/480 OD ratio DNA GS DNA library preparation for both shotgun and long paired-end DNA library preparation and sequencing reactions were performed at Research and Testing Laboratories (www.researchandtesting.com) following Genome Sequencer FLX System User’s instruction (Roche Diagnostics, Indianapolis, IN).

Submitted by: Medical Biofilm Research Institute (MBRI)

Study: Escherichia coli UM146 Genome sequencing

PRJNA50883 • SRP003159 • All experiments • All runs

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The MBRI has completed the genome sequence of the Adherent invasive <i>Escherichia coli</i> strain UM146, a highly invasive strain associated with ileal Crohn's disease.

Sample: Complete genome sequence of adherent invasive Escherichia coli UM146 isolated from ileal Crohn’s disease biopsy tissue

SAMN00017915 • SRS085789 • All experiments • All runs

Organism: Escherichia coli UM146

Library:

Name: EcoliUM146

Instrument: 454 GS FLX Titanium

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 225,673 spots, 118.8M bases, 248.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR062269	225,673	118.8M	248.8Mb	2011-02-18

ID:: 27135

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