GSM1215618: 7dpa; Danio rerio; RNA-Seq - SRA

SRX339605: GSM1215618: 7dpa; Danio rerio; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 32M spots, 6.4G bases, 4.2Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptome analysis of heart regeneration in adult zebrafish

PRJNA217180 • SRP029227 • All experiments • All runs

show Abstracthide Abstract

After heart injury, adult zebrafish can perfectly regenerate its heart without any scar tissue left. We performed 100bp*2, paired-end, strand-specific, polyA-positive RNA-seq on hearts from 2 group of adult zebrafish, in which one was sham group and the other 7 days after heart tip amputation (7dpa). We found pathways involving hydrogen peroxidate related functions significantly up-regulated in 7dpa group, indicating its role in heart regeneration. Overall design: RNA-seq: hearts from sham or 7dpa adult zebrafish

Sample: 7dpa

SAMN02334703 • SRS473371 • All experiments • All runs

Organism: Danio rerio

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: total RNA was extracted using QIAGEN RNeasy Microarray Tissue Mini Kit (Cat No. 73304) Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed; Exome fragments were captured and enriched by SureSelect Human All Exon 50 Mb kits (Agilent Technologies) following the manufacture’s protocol.

Experiment attributes:

GEO Accession: GSM1215618

Links:

External link: GEO Sample GSM1215618

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 32M spots, 6.4G bases, 4.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR957180	32,017,658	6.4G	4.2Gb	2015-07-22

ID:: 477374

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity