Genome sequencing of the holosporalean 'Candidatus Finniella inopinat... - SRA

SRX4966076: Genome sequencing of the holosporalean 'Candidatus Finniella inopinata'
1 ILLUMINA (Illumina MiSeq) run: 3M spots, 903.1M bases, 489.7Mb downloads

Design: DNA extraction of total gDNA from axenic cultures of Viridiraptor invadens strain Virl02 (the host of Candidatus Finniella inopinata) was carried out with the ZR Fungal/Bacterial DNA MicroPrep Kit (Zymo Research) using a BIO101/Savant FastPrep FP120 high-speed bead beater and 20 uL of proteinase K (20 mg/mL). A sequencing library was made using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). Culturing was done by Sebastian Hess (Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada). DNA isolation, sequencing, and assembly was performed by Sergio A. Munoz-Gomez (Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada).

Submitted by: Center for Comparative Genomics and Evolutionary Bioinformatics

Study: Sequencing of alphaproteobacterial endosymbionts that belong to the Rickettsiales and Holosporales orders.

PRJNA501864 • SRP167459 • All experiments • All runs

Sample:

SAMN10348102 • SRS4005585 • All experiments • All runs

Organism: Candidatus Finniella inopinata

Library:

Name: HOLO01

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM PCR

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 3M spots, 903.1M bases, 489.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR8145469	3,006,282	903.1M	489.7Mb	2018-11-30

ID:: 6695091

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