Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA from T. magnatum free-living mycelium and fruiting bodies was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich), following the protocol A and the on-column DNase Digestion procedure. Total RNA was extracted from ectomycorrhizal root tips of T. magnatum/Q.robur using the RNeasy Plant Mini Kit of Qiagen with DNase step and addition of 20 mg/ml polyethylene glycol to the RLC extraction buffer. cDNA libraries were prepared for sequencing using standard Illumina protocols by GeT (Génopole Toulouse Midi-Pyrénées, Auzeville, France)