Mock_Powerlyse_RNA-later_30_Platinum - SRA

SRX3425578: Mock_Powerlyse_RNA-later_30_Platinum
1 ILLUMINA (Illumina MiSeq) run: 206,939 spots, 103.9M bases, 64.7Mb downloads

Design: Mock community was stored in RNA-later and extracted with MOBIO PowerLyzer PowerSoil Kit . The V4 region of the 16S rRNA gene was amplified for 30 cycles using the Platinum SuperFi DNA Polymerase .

Submitted by: German Primate Center, Leibniz Institute for Primate Research

Study: Comparing different sample handling conditions for 16S rRNA amplicon sequencing

PRJNA420005 • SRP125723 • All experiments • All runs

show Abstracthide Abstract

We examined a combination of sample handling choices (sample storage, extraction kit and 16S amplification) for swab material using an even mock community (HM-280). In addition, blank controls were used to monitor the level of laboratory and kit-specific contamination. By examining a combination of effects, we aim to contribute to the ongoing discussion regarding standardization of sample handling for amplicon and metagenomic sequencing.

Sample: A cell mixture of 22 different bacterial strains at a concentration of 1x10^8 cells/mL of each organism (Microbial mock community HM-280) in phosphate buffer saline (PBS) from the Biodefense and Emerging Infectious Research (BEI) Resources of the American Type Culture Collection (ATCC) (Manassas, USA)

SAMN08103191 • SRS2717131 • All experiments • All runs

Organism: synthetic metagenome

Library:

Name: Mock_Powerlyse_RNA-later_30_Platinum

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 206,939 spots, 103.9M bases, 64.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR6325828	206,939	103.9M	64.7Mb	2017-11-28

ID:: 4769661

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