Mock_QMINI_LysisBuffer_30_Platinum - SRA

SRX3425591: Mock_QMINI_LysisBuffer_30_Platinum
1 ILLUMINA (Illumina MiSeq) run: 266,259 spots, 133.7M bases, 84Mb downloads

Design: Mock community was stored in LysisBuffer and extracted with QIAamp DNA Mini Kit (QMINI). The V4 region of the 16S rRNA gene was amplified for 30 cycles using the Platinum SuperFi DNA Polymerase .

Submitted by: German Primate Center, Leibniz Institute for Primate Research

Study: Comparing different sample handling conditions for 16S rRNA amplicon sequencing

PRJNA420005 • SRP125723 • All experiments • All runs

show Abstracthide Abstract

We examined a combination of sample handling choices (sample storage, extraction kit and 16S amplification) for swab material using an even mock community (HM-280). In addition, blank controls were used to monitor the level of laboratory and kit-specific contamination. By examining a combination of effects, we aim to contribute to the ongoing discussion regarding standardization of sample handling for amplicon and metagenomic sequencing.

Sample: A cell mixture of 22 different bacterial strains at a concentration of 1x10^8 cells/mL of each organism (Microbial mock community HM-280) in phosphate buffer saline (PBS) from the Biodefense and Emerging Infectious Research (BEI) Resources of the American Type Culture Collection (ATCC) (Manassas, USA)

SAMN08103191 • SRS2717131 • All experiments • All runs

Organism: synthetic metagenome

Library:

Name: Mock_QMINI_LysisBuffer_30_Platinum

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 266,259 spots, 133.7M bases, 84Mb

Run	# of Spots	# of Bases	Size	Published
SRR6325815	266,259	133.7M	84Mb	2017-11-28

ID:: 4769674

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