show Abstracthide AbstractThe focal male for shotgun sequencing (454 and Illumina small insert) was the son of a queen from a polygynous colony that was originally collected near Athens, Georgia, USA in 2008. The large-insert paired libraries were made from pools of the focal male''s brothers. All used individuals had the Gp-9b genotype. Upon transfer to the laboratory, the queen had been isolated with workers and reared as the solitary queen of a colony for one year to ensure that all progeny in the colony were hers.