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SRX2676984: GSM2551957: Tubae_Qrob_mycorrhiza 2; Quercus robur; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 38.1M spots, 11.4G bases, 4.3Gb downloads

Submitted by: NCBI (GEO)
Study: The transcriptome of Oak roots associated with truffles
show Abstracthide Abstract
Illumina HiSeq technology was used to generate mRNA profiles from mycorrhizal Quercus robur roots. Tuber melanosporum, T. aestivum and T.magnatum mycorrhizal root tips were harvested and used for RNA extraction. Paired-end reads of 100 bp were generated and aligned to Quercus robur CDS using CLC Genomics Workbench 9. Overall design: mRNA profiles from Tuber-Oak mycorrhizas were generated by Illumina HiSeq2000 sequencing (2x100bp). Three biological replicates were sequenced from Tuber melanosporum, T. aestivum and T.magnatum mycorrhizal root tips .
Sample: Tubae_Qrob_mycorrhiza 2
SAMN06647526 • SRS2075410 • All experiments • All runs
Organism: Quercus robur
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA of 100 mg of tissue per sample was extracted using RNeasy Plant Mini Kit (Qiagen). Quantification and integrity check were conducted using an Experion Automated Electrophoresis Station (Bio-Rad, Hercules, CA, USA). cDNA libraries were prepared for sequencing using standard Illumina protocols by GeT (Génopole Toulouse Midi-Pyrénées, Auzeville, France)
Experiment attributes:
GEO Accession: GSM2551957
Links:
Runs: 1 run, 38.1M spots, 11.4G bases, 4.3Gb
Run# of Spots# of BasesSizePublished
SRR538187138,096,26111.4G4.3Gb2018-07-02

ID:
3862024

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