GSM2551950: Tubmel_Qrob_mycorrhiza 1; Quercus robur; RNA-Seq - SRA

SRX2676977: GSM2551950: Tubmel_Qrob_mycorrhiza 1; Quercus robur; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 38.9M spots, 11.7G bases, 4.5Gb downloads

Submitted by: NCBI (GEO)

Study: The transcriptome of Oak roots associated with truffles

PRJNA380755 • SRP102584 • All experiments • All runs

show Abstracthide Abstract

Illumina HiSeq technology was used to generate mRNA profiles from mycorrhizal Quercus robur roots. Tuber melanosporum, T. aestivum and T.magnatum mycorrhizal root tips were harvested and used for RNA extraction. Paired-end reads of 100 bp were generated and aligned to Quercus robur CDS using CLC Genomics Workbench 9. Overall design: mRNA profiles from Tuber-Oak mycorrhizas were generated by Illumina HiSeq2000 sequencing (2x100bp). Three biological replicates were sequenced from Tuber melanosporum, T. aestivum and T.magnatum mycorrhizal root tips .

Sample: Tubmel_Qrob_mycorrhiza 1

SAMN06647524 • SRS2075404 • All experiments • All runs

Organism: Quercus robur

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA of 100 mg of tissue per sample was extracted using RNeasy Plant Mini Kit (Qiagen). Quantification and integrity check were conducted using an Experion Automated Electrophoresis Station (Bio-Rad, Hercules, CA, USA). cDNA libraries were prepared for sequencing using standard Illumina protocols by GeT (Génopole Toulouse Midi-Pyrénées, Auzeville, France)

Experiment attributes:

GEO Accession: GSM2551950

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 38.9M spots, 11.7G bases, 4.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR5381864	38,915,579	11.7G	4.5Gb	2018-07-02

ID:: 3862017

SRA

Sequence Read Archive

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