nifH amplicon sequencing of surface (5-200 m) water samples across a ... - SRA

SRX1950805: nifH amplicon sequencing of surface (5-200 m) water samples across a West to East transect(~14 °N) of the Tropical North Atlantic.
1 ILLUMINA (Illumina MiSeq) run: 112 spots, 48,291 bases, 107,489b downloads

Design: Extracted DNA samples were prepared for nifH amplicon sequencing using a nested PCR following established protocols (Zehr et al., 2011) with modifications as previously described ( Bentzon-Tilia et al., 2015). Illumina indices were added to amplicons in the second PCR round. In addition to the nifH1 and nifH2 primer sequences, primers contained a linker sequence, an 8-base barcode, and the Illumina specific region P5 (forward primer) or P7 (reverse primer). Refs: Zehr, J. P. & Turner, P. J. in Methods in Microbiology Vol. Volume 30 271-286 (Academic Press, 2001); Bentzon-Tilia, M. et al. Significant N2 fixation by heterotrophs, photoheterotrophs and heterocystous cyanobacteria in two temperate estuaries. The ISME journal 9, 273-285 (2015)

Submitted by: MPI for Marine Microbiology

Study: Raw sequence reads from Tropical North Atlantic surface water -nifH amplicons

PRJNA326820 • SRP078566 • All experiments • All runs

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Diversity of nitrogenase gene sequences in Atlantic waters

Sample: Metagenome or environmental sample from marine environment

SAMN05292969 • SRS1565251 • All experiments • All runs

Organism: marine metagenome

Library:

Name: CTD17_70m

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 112 spots, 48,291 bases, 107,489b

Run	# of Spots	# of Bases	Size	Published
SRR3924409	112	48,291	107,489b	2016-07-16

ID:: 2808628

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