Environmental partial nifH sequences from sample: NPSG_75m_S43 - SRA

SRX1943910: Environmental partial nifH sequences from sample: NPSG_75m_S43
1 ILLUMINA (Illumina MiSeq) run: 111,798 spots, 33.7M bases, 18.8Mb downloads

Design: Partial nifH sequences were amplified from environmental DNA using nested PCR with the universal primers of Zehr and McReynolds (1989) and Zani (2000). Samples were multiplexed using a one-step PCR with gene-specific sites, forward and reverse barcodes, Illumina linkers, and sequencing primer binding regions, similar to the method of Kozich et al. (2013). Though this was a paired-end run and both forward and reverse index reads were used for demultiplexing, only forward reads could be used for phylogenetic analyses due to the poor quality of reverse reads.

Submitted by: Oregon State University

Study: Comparing diazotrophic diversity among ocean regions

PRJNA328516 • SRP078449 • All experiments • All runs

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High throughput partial nifH sequences compared among oceanographic stations from the Eastern South Pacific, the North Pacific Subtropical Gyre, and the Pacific Northwest coast of the United States.

Sample: Partial nifH sequences from NPSG_75m_S43

SAMN05374892 • SRS1561651 • All experiments • All runs

Organism: marine metagenome

Library:

Name: NPSG_75m_S43_library

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 111,798 spots, 33.7M bases, 18.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR3898627	111,798	33.7M	18.8Mb	2016-07-18

ID:: 2800944

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Sequence Read Archive

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