RNA-seq of barcodes in barcoded rabies virus - SRA

SRX19253435: RNA-seq of barcodes in barcoded rabies virus
1 ILLUMINA (Illumina MiSeq) run: 833,351 spots, 100M bases, 50.3Mb downloads

Design: Rabies genome was extracted, reverse transcribed with an RT primer containing a N15 UMI, and then PCRed to add P5 and P7 binding sites

Submitted by: Allen Institute for Brain Science

Study: Barcoded rabies library

Barcoded rabies virus library used for barcoded rabies tracing using single-cell RNAseq and in situ sequencing.

Sample:

Library:

Name: RVdG-4mCherry_20-mer barcode(B19G)

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: VIRAL RNA

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 833,351 spots, 100M bases, 50.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR23310758	833,351	100M	50.3Mb	2023-04-17

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