WGS Dmel female A4xISO1 PacBio HiFi 24kb - SRA

SRX6957826: WGS Dmel female A4xISO1 PacBio HiFi 24kb
1 PACBIO_SMRT (Sequel II) run: 1.1M spots, 25.8G bases, 20.1Gb downloads

Design: gDNA was extracted according to Chakraborty et al (2016), Nucleic Acids Research 44:19 pp e147, from F1 females resulting from A4 females crossed to ISO1 male Drosophila melanogaster. DNA sheared on Diagenode Megaruptor 1 system using a 20 kb target. Library made following the PacBio protocol: Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0. Final library was size fractionated with Sage Science SageELF system. A 24kb fraction was sequenced on one Sequel II SMRT Cell 8M using Sequel II Sequencing Plate 2.0 and Sequel II Binding Kit 2.0. HiFi reads generated with CCS Analysis in SMRTLink v. 8.0.0 using default parameters.

Submitted by: PacBio

Study: Human, rice, and Drosophila HiFi datasets from PacBio Sequel II

PRJNA573706 • SRP223310 • All experiments • All runs

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PacBio HiFi (CCS) datasets collected for model systems using Sequel II system for studies of de novo genome assembly performance

Sample: Drosophila melanogaster F1 females from A4 X ISO1 cross

SAMN12476704 • SRS5269936 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Name: Dmel_A4xISO1_HiFi_24kb

Instrument: Sequel II

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 1.1M spots, 25.8G bases, 20.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR10238607	1,057,390	25.8G	20.1Gb	2019-10-07

ID:: 9154820

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