Forward Reads - SRA

SRX7719436: Forward Reads
1 ILLUMINA (Illumina MiniSeq) run: 630,544 spots, 189.2M bases, 111.6Mb downloads

Design: The pADL-10b plasmid was isolated from E. coli samples that were used for phage expression. Primers specific to the library region of the pADL-10b plasmid were then used to create amplicons of the library region. Two subsequent PCR steps were then performed to attach Illumina adapters and indices that allowed for Illumina sequencing of the amplicons.

Submitted by: Texas A&M University

Study: Generation of Amber Obligate Phage Library

PRJNA606283 • SRP249335 • All experiments • All runs

show Abstracthide Abstract

In this study, we aim to develop amber obligate phage libraries that will allow for efficient incorporation of unnatural amino acids into phage libraries via amber suppression. Analysis of this amber obligate library was done through Illumina sequencing of amplicons of the phage library on pIII.

Sample:

SAMN14088869 • SRS6141743 • All experiments • All runs

Organism: Inovirus M13

Library:

Name: 1_S1_L001_R1_001

Instrument: Illumina MiniSeq

Strategy: AMPLICON

Source: SYNTHETIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 630,544 spots, 189.2M bases, 111.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR11080384	630,544	189.2M	111.6Mb	2020-02-13

ID:: 10090266

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