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SRX10986853: Illumina HiSeq 2500 genome sequencing of Xanthomonas campestris: DAR-41284
1 ILLUMINA (Illumina HiSeq 2500) run: 3.1M spots, 785.5M bases, 325.4Mb downloads

Design: Xanthomonas DNA was extracted using the DNeasy Blood and Tissue kit (QIAGEN) according to the manufacturers instructions. For each sample, tagmentation of genomic DNA, and PCR amplification of tagged DNA were performed in triplicate using the Nextera XT system (Illumina). Sequencing libraries were pooled, then cleaned and size selected using SPRI beads (Beckman Coulter). Normalization was guided by read counts obtained from a Nano flowcell run on a MiSeq instrument (Illumina). Agilent 2100 Bioanalyzer, with High Sensitivity DNA kit was used to quantitate the pooled library before loading onto an Illumina HiSeq. Paired-end 150 bp reads were generated using the HiSeq 2500 system.
Submitted by: NSW Department of Primary Industries
Study: Comparaitve genomics of Xanthomonas campestris isolates
PRJNA693392 • SRP321303 • All experiments • All runs
show Abstracthide Abstract
Xanthomonas campestris is the cause of several commercially important diseases of Brassica spp. To further knowledge of this important pathogen we sequenced the genomes of 261 X. campestris isolates from NSW Department of Primary Industries archives and international culture collections.
Sample:
SAMN17385932 • SRS9061445 • All experiments • All runs
Organism: Xanthomonas campestris
Library:
Name: DAR-41284.illumina.uts
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 3.1M spots, 785.5M bases, 325.4Mb
Run# of Spots# of BasesSizePublished
SRR146482813,141,821785.5M325.4Mb2024-06-29

ID:
14608247

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