Illumina MiSeq sequencing; qiita_ptid_5704:10246.HI.B1 - SRA

ERX2886835: Illumina MiSeq sequencing; qiita_ptid_5704:10246.HI.B1
1 ILLUMINA (Illumina MiSeq) run: 55,235 spots, 8.3M bases, 4.8Mb downloads

Design: We collected soil samples along two arctic transects in North America, NAAT and Eurasia, EAT. Each transect extends across all five bioclimatic subzones of the Arctic.

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: The North American Arctic Transect, NAAT and the Eurasian Arctic Transect, EAT

PRJEB29570 • ERP111883 • All experiments • All runs

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We collected soil samples along two arctic transects in North America, NAAT and Eurasia, EAT. Each transect extends across all five bioclimatic subzones of the Arctic.

Sample: Timling_arctic_transects; 10246.HI.B1

SAMEA5072024 • ERS2883392 • All experiments • All runs

Organism: soil metagenome

Library:

Name: 10246.HI.B1

Instrument: Illumina MiSeq

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: Illumina EMP protocol 515fbc, 806r amplification of 16S rRNA V4

Experiment attributes: (show all 29 attributes...) (hide...)

barcode: TTACACAAAGGC

center_name: UCSDMI

center_project_name: EMP-Timling

extraction_robot: NA

extractionkit_lot: NA

linker: GT

mastermix_lot: 620338

orig_name: HI.B1

pcr_primers: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT

plating: NA

primer: GTGTGYCAGCMGCCGCGGTAA

primer_date: 73118

primer_plate: 1

processing_robot: ROBE

project_name: Arctic Transects Soils

run_center: UCSDMI

run_date: 9/26/18

run_prefix: Arctic_Transects_Soils_1-3_Prather_Seawater_SU18_1 (show full text...)(hide...)

Arctic_Transects_Soils_1-3_Prather_Seawater_SU18_1_S1_L001

runid: 180926_M05314_0126_000000000-C4PNL

sample_plate: Arctic_Transects_Soils_Plate_1

sequencing_meth: Sequencing by synthesis

target_gene: 16S rRNA

target_subfragment: V4

tm1000_8_tool: NA

tm300_8_tool: 208484Z

tm50_8_tool: 108364Z

water_lot: RNBG7049

well_description: Arctic_Transects_Soils_Plate_1_HI.B1_F9

well_id: F9

Runs: 1 run, 55,235 spots, 8.3M bases, 4.8Mb

Run	# of Spots	# of Bases	Size	Published
ERR2881886	55,235	8.3M	4.8Mb	2018-11-09

ID:: 6738086

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