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ERX1845447: Illumina HiSeq 2000 sequencing; qiita_ptid_280:1683.UT15.52a
1 ILLUMINA (Illumina HiSeq 2000) run: 1.7M spots, 154.3M bases, 105.8Mb downloads

Design: comparison of samples run on HiSeq and MiSeq; these are HiSeq
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
show Abstracthide Abstract
Twenty-four samples were sequenced on three paired-end Illumina HiSeq2000 lanes, and in one paired-end MiSeq run. The samples represented soil (source: USA; n=8) and several host-associated environment types: human feces (source: USA; n=2), mouth (source: USA; n=2) and skin (source: USA; n=6); canine feces (source: USA; n=1) mouth (source: USA; n=1) and skin (source: USA; n=4). These four paired-end lanes (three on HiSeq and one on MiSeq) resulted in eight sets of reads, corresponding to 5 and 3-prime reads from each lane. These sets of reads were treated as independent replicates to assess the reproducibility of the results.
Sample: 1683.UT15.52a; Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
SAMEA46811668 • ERS1500530 • All experiments • All runs
Organism: soil metagenome
Library:
Name: 1683.UT15.52a
Instrument: Illumina HiSeq 2000
Strategy: OTHER
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: Illumina V4 MiSeq 515/806 primers
Experiment attributes: (show all 17 attributes...) (hide...)
barcode: AGGACGCACTGT
center_name: CCME
center_project_name: Caporaso_IlluminaComp_HiSeq
experiment_center: CCME
experiment_title: Ultra-high-throughput microbial community analysis (show full text...)(hide...)
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
illumina_technology: HiSeq
linker: GT
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer: GTGCCAGCMGCCGCGGTAA
run_center: CCME
run_date: 3/29/12
run_prefix: s_6_1_sequences
samp_size: .1,g
sample_center: CCME
sequencing_meth: sequencing by synthesis
target_gene: 16S rRNA
target_subfragment: V4
Runs: 1 run, 1.7M spots, 154.3M bases, 105.8Mb
Run# of Spots# of BasesSizePublished
ERR17817241,720,908154.3M105.8Mb2017-01-14

ID:
3615048

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