Illumina HiSeq 2000 sequencing; qiita_ptid_77:678.T.RFLP.Sample.655 - SRA

ERX1723848: Illumina HiSeq 2000 sequencing; qiita_ptid_77:678.T.RFLP.Sample.655
1 ILLUMINA (Illumina HiSeq 2000) run: 27,928 spots, 3.9M bases, 2.9Mb downloads

Design: Samples taken from surface sediments and sediment from the burrow walls of Upogebia deltaura, a bioturbating shrimp species. Sediments were subjected to 5 different pH levels over a 14-week exposure period. Bacterial and archaeal 16S genes were sequenced.

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

PRJEB15461 • ERP017221 • All experiments • All runs

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Investigation into the effects of ocean acidification on microbial community structure and diversity in bioturbated coastal marine sediments. Samples taken from surface sediments and sediment from the burrow walls of Upogebia deltaura, a bioturbating shrimp species. Sediments were subjected to 5 different pH levels over a 14-week exposure period. Bacterial and archaeal 16S genes were sequenced.

Sample: Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments; 678.T.RFLP.Sample.655

SAMEA4460960 • ERS1360139 • All experiments • All runs

Organism: marine sediment metagenome

Library:

Name: 678.T.RFLP.Sample.655

Instrument: Illumina HiSeq 2000

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: EMP V4 515f,806rbc protocol

Experiment attributes: (show all 18 attributes...) (hide...)

barcode: GACTGACTCGTC

center_name: Plymouth Marine Laboratory

center_project_name: Investigation into the effects of ocean acidificat (show full text...)(hide...)

Investigation into the effects of ocean acidification on microbial communities in bioturbated coastal sediments

emp_status: EMP

illumina_technology: HiSeq

linker: GT

orig_sample_name: 655

pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

primer: GTGTGCCAGCMGCCGCGGTAA

run_center: ANL

run_date: 11/3/10

run_prefix: Bonnie-Pool-and-1percentPhiX_110310_HWUSI-EAS552R_ (show full text...)(hide...)

Bonnie-Pool-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_5_1_sequence

samp_size: .1,g

sample_center: ANL

sequencing_meth: sequencing by synthesis

study_center: CCME

target_gene: 16S rRNA

target_subfragment: V4

Runs: 1 run, 27,928 spots, 3.9M bases, 2.9Mb

Run	# of Spots	# of Bases	Size	Published
ERR1653532	27,928	3.9M	2.9Mb	2016-10-05

ID:: 3247054

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