Illumina HiSeq 2000 sequencing; qiita_ptid_77:678.OA.mesocosm.392 - SRA

ERX1723759: Illumina HiSeq 2000 sequencing; qiita_ptid_77:678.OA.mesocosm.392
1 ILLUMINA (Illumina HiSeq 2000) run: 107,805 spots, 15M bases, 11.2Mb downloads

Design: Samples taken from surface sediments and sediment from the burrow walls of Upogebia deltaura, a bioturbating shrimp species. Sediments were subjected to 5 different pH levels over a 14-week exposure period. Bacterial and archaeal 16S genes were sequenced.

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

PRJEB15461 • ERP017221 • All experiments • All runs

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Investigation into the effects of ocean acidification on microbial community structure and diversity in bioturbated coastal marine sediments. Samples taken from surface sediments and sediment from the burrow walls of Upogebia deltaura, a bioturbating shrimp species. Sediments were subjected to 5 different pH levels over a 14-week exposure period. Bacterial and archaeal 16S genes were sequenced.

Sample: Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments; 678.OA.mesocosm.392

SAMEA4460871 • ERS1360050 • All experiments • All runs

Organism: marine sediment metagenome

Library:

Name: 678.OA.mesocosm.392

Instrument: Illumina HiSeq 2000

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: EMP V4 515f,806rbc protocol

Experiment attributes: (show all 18 attributes...) (hide...)

barcode: TCTACGGCACGT

center_name: Plymouth Marine Laboratory

center_project_name: Investigation into the effects of ocean acidificat (show full text...)(hide...)

Investigation into the effects of ocean acidification on microbial communities in bioturbated coastal sediments

emp_status: EMP

illumina_technology: HiSeq

linker: GT

orig_sample_name: 392

pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

primer: GTGTGCCAGCMGCCGCGGTAA

run_center: ANL

run_date: 11/3/10

run_prefix: Bonnie-Pool-and-1percentPhiX_110310_HWUSI-EAS552R_ (show full text...)(hide...)

Bonnie-Pool-and-1percentPhiX_110310_HWUSI-EAS552R_0357_s_5_1_sequence

samp_size: .1,g

sample_center: ANL

sequencing_meth: sequencing by synthesis

study_center: CCME

target_gene: 16S rRNA

target_subfragment: V4

Runs: 1 run, 107,805 spots, 15M bases, 11.2Mb

Run	# of Spots	# of Bases	Size	Published
ERR1653443	107,805	15M	11.2Mb	2016-10-05

ID:: 3246965

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