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ERX1664856: Illumina HiSeq 2000 sequencing; qiita_ptid_55:1721.S28B
1 ILLUMINA (Illumina HiSeq 2000) run: 18,362 spots, 2.6M bases, 1.8Mb downloads

Design: This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time.
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: A combination of biochar-mineral complexes and compost improves soil bacterial processes, soil quality, and plant properties
show Abstracthide Abstract
Biochar is derived from the oxygen-limited pyrolysis of organic matter (e.g. agricultural or municipal waste) and constitutes for complex structure of recalcitrant, porous carbon and well as mobile minerals and nutrients. Several studies have shown that the addition of biochar improves agricultural yields and performance in a way comparable to classical NPK or urea-based fertilisers. Biochar is therefore an emerging and attractive replacement for classical farming practices with the additional benefit of sequestering carbon that would otherwise be burnt or composted. The mechanisms behind the beneficial properties of biochar are not well understood, but initial work has shown that its amendment to soil impacts on microbial community composition. To better understand these processes, this project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time.
Sample: A combination of biochar-mineral complexes and compost improves soil bacterial processes, soil quality, and plant properties; 1721.S28B
SAMEA4393056 • ERS1304505 • All experiments • All runs
Organism: soil metagenome
Library:
Name: 1721.S28B
Instrument: Illumina HiSeq 2000
Strategy: OTHER
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: EMP V4 515f,806rbc protocol
Experiment attributes: (show all 20 attributes...) (hide...)
barcode: CTATCCAAGTGG
center_name: CCME
center_project_name: Thomas_barley_soil
emp_status: EMP
illumina_technology: HiSeq
linker: GT
location: SG84
number: 227.0
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
plate: Soil
primer: GTGTGCCAGCMGCCGCGGTAA
run_center: CCME
run_date: 3/1/14
run_prefix: Thomas_barley_soil_lane5_NoIndex_L005
samp_size: 0.1, gram
sample_center: Thomas Lab, Sydney, Australia
sequencing_meth: sequencing by synthesis
study_center: CCME
target_gene: 16S rRNA
target_subfragment: V4
Runs: 1 run, 18,362 spots, 2.6M bases, 1.8Mb
Run# of Spots# of BasesSizePublished
ERR159427018,3622.6M1.8Mb2016-08-29

ID:
3045447

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