Illumina HiSeq 2000 sequencing; qiita_ptid_59:1747.slasher.29.101112 - SRA

ERX1545573: Illumina HiSeq 2000 sequencing; qiita_ptid_59:1747.slasher.29.101112
1 ILLUMINA (Illumina HiSeq 2000) run: 86,888 spots, 13.1M bases, 7.9Mb downloads

Design: characterization of komodo dragon microbiome from zoo specimens

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: The oral and skin microbiomes of captive Komodo Dragons are significantly shared with their habitat.

PRJEB14602 • ERP016252 • All experiments • All runs

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Here we collect saliva, skin, and fecal samples from captive Komodo dragons (V. komodoensis) and other captive varanids (V. olivaceus, V. indicus, V. rudicollis) in the first-ever culture-independent assessment of the varanid microbiome. Environmental samples were also collected to characterize host-environment microbiome sharing. This study adds crucial information to our understanding of captive Komodo dragon microbial ecology.

Sample: 1747.slasher.29.101112; Development of the oral microbiota in captive Komodo Dragons (Varanus komodoensis)

SAMEA4051518 • ERS1222628 • All experiments • All runs

Organism: skin metagenome

Library:

Name: 1747.slasher.29.101112

Instrument: Illumina HiSeq 2000

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: EMP V4 515f,806rbc protocol

Experiment attributes: (show all 17 attributes...) (hide...)

barcode: GTTGTTCTGGGA

center_name: CCME

center_project_name: Knight_komodo

emp_status: EMP

illumina_technology: HiSeq

linker: GT

pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

primer: GTGTGCCAGCMGCCGCGGTAA

run_center: CCME

run_date: 3/4/13

run_prefix: KomodoDragon_16sV4_L001_sequences

samp_size: .1,g

sample_center: CCME

sequencing_meth: sequencing by synthesis

study_center: CCME

target_gene: 16S rRNA

target_subfragment: V4

Runs: 1 run, 86,888 spots, 13.1M bases, 7.9Mb

Run	# of Spots	# of Bases	Size	Published
ERR1474426	86,888	13.1M	7.9Mb	2016-06-28

ID:: 2691299

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