Illumina MiSeq sequencing; 16s rRNA-young and old mouse gut microbiot... - SRA

ERX6137241: Illumina MiSeq sequencing; 16s rRNA-young and old mouse gut microbiota-stroke
1 ILLUMINA (Illumina MiSeq) run: 74,435 spots, 18.7M bases, 10.7Mb downloads

Design: 16s rRNA-young and old mouse gut microbiota-stroke

Submitted by: UNIVERSITY OF VIRGINIA

Study: 16s rRNA-young and old mouse gut microbiota-stroke

PRJEB47036 • ERP131275 • All experiments • All runs

show Abstracthide Abstract

To explore the effects of gut microbiota of young (8 weeks) or old mice (18~20 months) on stroke, feces of young (Y1-Y9) and old mice (O6-O16) were collected and analyzed by 16s rRNA sequencing. Then stroke model was established on young mouse receive feces from old mouse (DOT1-15) and young mouse receive feces from young mouse (DYT1-15). 16s rRNA sequencing were also performed for those young mice received feces from young and old mice.

Sample: DYT14

SAMEA9699278 • ERS7423510 • All experiments • All runs

Organism: Mus musculus

Library:

Name: DYT14_s

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: Stroke model: Old or young C57BL/6J male mice were subjected to the left cerebral artery occlusion (MCAO), this surgery will cause ischemic stroke in mice. Mouse feces were harvested and stored at -80Â°C until further use. DNA was extracted per the manual of Power Lyzer Power soil DNA isolation kit (QIAGEN). per the protocol of \"16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System\".

Experiment attributes:

Experimental Factor: age: 2

Experimental Factor: treatment: fecal transfer from young mouse

Runs: 1 run, 74,435 spots, 18.7M bases, 10.7Mb

Run	# of Spots	# of Bases	Size	Published
ERR6510356	74,435	18.7M	10.7Mb	2021-10-19

ID:: 17181766

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