Whole genome sequencing of Mycoplasma pneumoniae strain 309 - SRA

DRX001134: Whole genome sequencing of Mycoplasma pneumoniae strain 309
1 LS454 (454 GS Junior) run: 151,617 spots, 88.3M bases, 178.7Mb downloads

Design: Mycoplasma pneumoniae strain 309 was cultured in 20 ml of PPLO medium at 37˚C for 5 days. M. pnenmoniae cells attached to the inner surface of the culture flask were scraped out and washed with phosphate buffered saline (PBS) twice. Cells were concentrated by centrifuge and suspended in 0.2 ml of PBS. Genomic DNA was extracted from cells using QIAamp DNA Mini Kit. Extracted DNA (26 ng/ micro liter) was diluted and sheared by Covaris S220 system according to manufacture’s instructions. Sheared DNA was purified by MinElute PCR Purification Kit and subjected to library preparation and 454 pyrosequencing according to manufacture’s instructions.

Submitted by: National Institute of Infectious Diseases - Japan (NIID)

Study: Complete genome sequencing of Mycoplasma pneumoniae strain 309

PRJDB8 • DRP000504 • All experiments • All runs

show Abstracthide Abstract

M. pneumoniae 309 is a type 2a strain of this bacterium. Type 2a strain was rare in 1990s but is frequently found in clinical specimens after 2002 in Japan. This strain has variations in cytadhesin protein P1, which is the essential factor for pathogenesis. This variation is probably created by recombination events between p1 gene and repetitive sequences in the genome. This mechanism could be clearly understood by complete genome sequencing of 309 strain. The precise comparison of complete M. pneumoniae genomes also detect the differences of surface antigenic protein composition between 309 and other strains. This is the key information to understand the clinical emergence of type 2a strain.

Sample: Whole genome sequencing of Mycoplasma pneumoniae strain 309

SAMD00012611 • DRS001148 • All experiments • All runs

Organism: Mycoplasmoides pneumoniae 309

Library:

Name: Mycoplasma pneumoniae strain 309 genomic DNA

Instrument: 454 GS Junior

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

5 forward

Pipeline: show...hide...

Step	Prev Step
1	NIL

Runs: 1 run, 151,617 spots, 88.3M bases, 178.7Mb

Run	# of Spots	# of Bases	Size	Published
DRR001648	151,617	88.3M	178.7Mb	2011-12-26

ID:: 128838

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity