SRA

There is growing recognition that mammalian genomes produce huge number of long noncoding RNAs (ncRNAs). However, the biological functions of most long ncRNAs are still unclear. Here we report a new approach to uncover the discriminative property of the functional long ncRNAs by combination of comprehensively measuring the half-lives and functional analysis. Our approach reveals hundreds of short-lived ncRNAs in which many well-known functional long ncRNAs are sorted. Moreover, we provide strong evidence that modulation of half-lives of relatively short-lived functional long ncRNAs, viz. NEAT1/MEN ? and GAS5, alters functional consequence, and identified novel short-lived intergenic noncoding transcripts (SLITs), those degradations are regulated by stimuli, involving in cell proliferation. Together, these results propose that short-lived property of ncRNA will be key parameter for identifying the uncharacterized long ncRNAs.