SRX11758600: Raw data after adapter removal from Nanopore long-read sequencing:Cas12a-mediated buf1 mutant:Rep1-buf1#5
1 OXFORD_NANOPORE (MinION) run: 237,771 spots, 2.8G bases, 2.4Gb downloads
Design: The DNA extractions for long-read nanopore sequencing were performed followed the online protocol (https://www.protocols.io/view/high-quality-dna-from-fungi-for-long-read-sequenci-k6qczdw). g-Tube (Covaris, catalog#520079) was used for shearing high-molecular-weight DNA into 20kb followed by AMPure XP beads purification (Beckman, catalog#NC9959336). The nanopore sequencing library preparation followed Native barcoding genomic DNA (with EXP-NBD104, EXPNBD114, and SQK-LSK109) protocol (https://community.nanoporetech.com/protocols/native-barcoding-genomic-dna/checklist_example.pdf).
Submitted by: Kansas state University
Study:
CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzaeshow Abstracthide AbstractDe novo genome assemblies of Cas12a-edited Magnaporthe oryzae buf1 and bas4 mutants based on nanopore long-read sequencing
Library:
Name: Rep1-buf1#5
Instrument: MinION
Strategy: WGS
Source: GENOMIC
Selection: other
Layout: SINGLE
Runs:
1 run, 237,771 spots, 2.8G bases, 2.4Gb